A restriction enzyme,Bpulis an isoschizomer ofBanll
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چکیده
منابع مشابه
A restriction endonuclease (DpaI) from Deleya pacifica IAM 14115, a marine bacterium, an isoschizomer of ScaI.
physicochemical properties. Deleya pacifica (Baumann et al. 1972) Baumann et al. 1983 IAM 141 15*1 was aerobically grown for 24hr at 30°C in a modified marine broth as described previously.2) The cells (30g) harvested from 6liters of culture broth by centrifugation were suspended in 200ml of a sonication buffer (pH 7.5) containing 10mM K2HPO4, 7mM 2-mercaptoethanol, 100mM NaCl and 20/xg/ml phen...
متن کاملIsolation and identification of the restriction endonuclease PtaI from Phormidium tadzschicicum, an isoschizomer of BspMII.
Ptal is a type II restriction endonuclease from the Cyanobacterial strain Phormidium tadzschicicum. This strain was obtained from the Collection of Algal Cultures of Leningrad University. Ptal recognizes the sequence 5'-TCCGGA-3' and cleaves between T and C. The enzyme was purified using the following chromatographic steps: 1) Phosphocelulose, 2) DEAESephadex. The enzyme was free of contaminati...
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The cold-active restriction endonuclease YenI, an isoschizomer of PstI, was found in 12 of 14 Yersinia enterocolitica serotype O8 strains of different origins, but not in other serotypes of Y. enterocolitica, Yersinia pseudotuberculosis, or Yersinia pestis. In spite of the limited number of strains tested, the result suggests that the detection of YenI endonuclease or the gene might result in m...
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We have purified RsrI endonuclease (R.RsrI), an isoschizomer of EcoRI, from Rhodobacter sphaeroides strain 630. The enzyme is homogeneous as judged by polyacrylamide gel electrophoresis and size-exclusion high-performance liquid chromatography. RsrI endonuclease is a dimer over the concentration range of 0.05 to 1.4 mg/ml. The reduced and denatured molecular weight of the enzyme is 30,000 Da. R...
متن کاملCharacterization of restriction endonuclease BdiSI, an isoschizomer of SfeI, from Bacteroides distasonis strain S-7.
weight), cultivated anaerobically at 37°C for 24hr in a medium containing 0.4% casamino acids, 0.3% yeast extract, 0.3% peptone, 1.0% glucose, 0.15% sodium chloride, 0.1% Tween 80 and 0.04% L-cysteine, pH 7.2, were disrupted by passing through a French pressure cell (Ohtake Works Co.) at 1500-1700kg/cm2. Nucleic acids were removed from the cell extracts by 1%streptomycin treatment, and then the...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.21.5850